Diversity and Safety Aspects of Coagulase-Negative Staphylococci in Ventricina del Vastese Italian Dry Fermented Sausage

Ventricina del Vastese is a traditional dry fermented sausage from Central Italy not yet characterized for the occurrence, identity and safety of coagulase-negative staphylococci (CNS), bacterial group technologically important this kind product. Therefore, in study, 98 CNS isolates four manufacturers were differentiated using repetitive element palindromic PCR (Rep-PCR) identified 16S rRNA gene sequencing. These examined genes encoding biogenic amine (BA) production, resistance to aminoglycosides, β-lactams, tetracyclines staphylococcal enterotoxins (SEs). Staphylococcus succinus (55%) predominated, followed by S. xylosus (30%), epidermidis (7.4%), equorum (3.1%), saprophyticus (3.1%) warneri (1%). One subsp. casei isolate was slightly β-hemolytic. SEs histidine decarboxylase hdcA detected, whereas tyrosine tdcA detected isolates. The blaZ beta-lactamase an isolate, tetracycline tetK six tetA one also bearing found. product peculiar species ratio low occurrence diversity AR transferable than found other studies, as probable consequence production only with meat animals raised small farms extensive rearing systems which antibiotic usage infrequent.